chromatography a method of sorting, analyzing and researching a mixture of substances , based on multiple sorption and desorption of components. The material from the mobile phase (solvent, solution) is sorbed on the stationary phase (sorbent). Chromatography is performed in a column (a tube filled with a sorbent), on paper (the solution moves through a sheet of paper – a sorbent) or in thin layers of sorbent powder. According to the aggregate state of the mobile phase, chromatography is divided into gas and liquid chromatography.
According to the mechanism of action, chromatography is: ion exchange (based on the unequal ability of the solution ions to replace the ionite ions in the column), exclusive (based on the distribution of molecules of unequal dimensions between the pore and moving solvent), precipitation (based on the different solubility of the precipitate), affinity, affinity, or biospecific ( based on the interaction of biologically active substances with the ligands of the sorbent), adsorptive (based on the interaction of the components of the test substance and the sorbent), partitioning (based on the unequal dissolution of the components in the sorbent).
Identified by chromatography
Many chemical compounds and natural products are separated and identified by chromatography methods: amino acids, proteins, hormones, oil, peptides, vitamins. The mixture is dissolved in a solvent and then passed through a medium, such as a column, that allows the different components to move at different rates. The different velocities are due to the different interactions of the components with the medium. The components can then be assembled and analyzed.
Chromatography can be used to separate and analyze a variety of molecules, including proteins, nucleic acids, and small molecules. It can be used to purify a mixture or to identify the components of a mixture. Chromatography can be performed using a variety of methods, including gas chromatography, liquid chromatography, and ion chromatography.
What is the basic principle of chromatography?
The basic principle of chromatography is that different molecules interact differently with the stationary and mobile phases. This interaction causes the molecules to travel at different speeds through the chromatography column. The molecules then separate according to their size, charge and other properties.
What is Sample Chromatography?
Chromatography is a process used to separate and analyze the components of a mixture. The mixture is placed on the porous material and the different components travel through the pores at different speeds. The different components can then be collected and analyzed.
One common type of chromatography is thin layer chromatography (TLC). In TLC, a mixture is placed on a thin layer of material and different components travel up the layer at different rates. The different components can then be collected and analyzed.
Another common method of chromatography is column chromatography. In column chromatography, a mixture is placed in a column of material and different components travel through the column at different speeds. Different components can then be collected and analyzed.
What are the 4 types of chromatographys?
- Paper chromatographys
- Gel filtration chromatographys
- Ion exchange chromatographys
- reverse phase chromatographys
What is the main purpose of chromatographys?
The main purpose of chromatographys is to separate the different components of a mixture so that they can be analyzed separately. This is done by passing the mixture through a medium, such as a column of adsorbent material, which interacts differently with each component. Different components travel through the medium at different speeds, so they can be separated. Electrophoresis is another member of this group. It is capable of separating all the components of a multicomponent chemical mixture without requiring an extensive foreknowledge of the identity, number, or relative amounts of the substances present.